US researchers say they
have created a "virtual" model of all the biochemical reactions
that occur in human cells.
They hope the computer model will allow scientists to
tinker with metabolic processes to find new treatments for
conditions such as high cholesterol.
It could also be used to individually tailor diet for
weight control, the University of California team claimed.
Their development is reported in the journal, Proceedings
of the National Academy of Sciences.
A team of six bioengineering researchers at the University
of California analysed the human genome to see what genes
corresponded to metabolic processes, such as those responsible
for the production of enzymes.
They spent a year manually going through 1,500 books,
review papers and scientific reports from the past 50 years
before constructing a database of 3,300 metabolic reactions.
The information was then used to create a network of metabolic
processes in the cell, similar to a traffic network.
Study leader Professor Bernhard Palsson said the network
could be used to see what would happen if a drug was used
to target a specific metabolic reaction, such as the synthesis
of cholesterol.
Or it could be used to predict what would happen if you
interfere with a metabolic reaction in a specific type of
cell, such as a blood or heart cell.
And eventually it could even be used to create an individual
network for a person.
"The new tool we've created allows scientists to tinker
with a virtual metabolic system in ways that were, until
now, impossible, and to test the modelling predictions in
real cells," said Mr Palsson, who is professor of bioengineering
and medicine.
"You can take a drug target and you can make the flow
through that reaction more and more restrictive or you can
calculate all the reactions that you have to go through
to make a certain product."
Metabolism
Metabolic reactions in cells include those which convert
food sources, such as fats, protein and carbohydrate into
energy and to make other molecules used by the body.
There are hundreds of human disorders which are a result
of problems with metabolism.
One example is haemolytic anaemia, a condition where red
blood cells are broken down too rapidly.
To test the computer model, the team ran 288 different
simulations, such as the synthesis of hormones, testosterone
and oestrogen, and the metabolism of fat from the diet.
"We all have natural variation in the capacity of these
pathways, for example in our ability to make cholesterol,
so you could make a metabolic model for an individual person
which is a tantalising prospect."
Keith Frayn, professor of human metabolism at the University
of Oxford, said the model would allow scientists to spot
potential problems with targeting certain reactions early
on in their research.
"It's increasingly recognised there are these networks
of metabolism and we need to know if we target something
how that will spread out and this is potentially a way of
dealing with that."
Dr Anthony Wierzbicki, consultant in specialist laboratory
medicine at St Thomas's hospital, has done a lot of work
on the role of cholesterol in heart disease.
"This is a potentially interesting tool for investigating
metabolism of which cholesterol biochemistry forms a part,"
he said.
But he added that the model would have to be "sophisticated"
enough to predict what happens in the production and breakdown
of cholesterol as well how it is absorbed from the gut as
the two were closely linked.